This microcourse covers common sources of shifts between images of different wavelengths (e.g., when imaging multiple fluorophores in the same specimen.

Some additional info:
Waters, J.C. (2009) Accuracy and precision in quantitative fluorescence microscopy. J. Cell Biol. 185, 1135–1148
Ross, S.T. et al. (2014) Practical considerations of objective lenses for application in cell biology. Methods Cell Biol. 123, 19–34
Manders, E.M.M. (1997) Chromatic shift in multicolour confocal microscopy. J. Microsc. 185, 321–328

Thank you to Talley Lambert, Rylie Walsh, Federico Gasparoli & Anna Jost for their feedback prior to release!